A RealTime PCR based multiplex assay to detect 22 pathogens causing Central Nervous System infections: diagnosis within 2.5 hours. Central nervous system (CNS) infections, such as meningitis and encephalitis can cause irreversible neurological damage, may be life-threatening and are therefore classified as medical emergencies. Causative agents cannot be identified from clinical symptoms and include a wide range of viruses, bacteria and fungi. Rapid and correct pathogen identification is important for timely and targeted therapy and clinical management. The MeningoFinder® 2SMART is a multiplex PCR test which can detect and differentiate 22 pathogens involved in CNS infections in a single assay using Melting Curve Analysis on Real Time PCR instruments.