Fast system for detection and genotyping of Human Papillomavirus by single-step PCR and reverse line blot
Fast system for detection and genotyping of Human Papillomavirus by Single-Step PCR and Reverse Line Blot
Allows identification of 40 HPV genotypes:
HPV 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68 (68a e 68b), 69, 70, 71, 72, 73, 81, 82, 83, 84, 87, 89, 90. HPV genotypes not listed above are detected by the Universal HPV sequence.
The PCR step employs the dUTP/UNG system for prevention of carry-over contamination.
The strip contains a TST band that detects a fragment of the housekeeping gene TST, which is amplified in multiplex with the HPV sequences. This amplification control (internal control) allows the user to assess the quality of the DNA extract.
The kit includes:
-A ready-for-use complete PCR master mix: only add DNA to an aliquot of the master mix
-An internal control: coamplification of a housekeeping gene
-A positive control: plasmid DNA containing a part of HPV 61
-Strips and reagents for visualization of HPV genotypes: analysis of the PCR product by Reverse Line Blot
INFORMATIONS FOR ORDERS
Code Description Format
03-35A-20 AMPLIQUALITY HPV-TYPE EXPRESS v3.0